As the majority of emerging and re-emerging human diseases are zoonotic in nature, monitoring wildlife health is a practical means for early identification of disease events. Surveillance in wild populations presents several logistical difficulties. Many of these obstacles can be overcome by using zoological institutions as disease sentinels. Zoos have a stable population of animals whose health is regularly monitored and recorded. This provides a wealth of valuable medical information and allows for serial sampling of individuals and groups over time. Additionally, zoos host a broad range of species, each with their own susceptibility to different maladies. This translates into the capacity to test for a wide array of disease agents. Another advantage zoos offer is their geographic distribution. With over 200 accredited institutions, zoos can be found in 47 states, spanning urban, suburban, and rural environments across the country. Last, as a site of increased human-animal interaction, zoos provide a unique opportunity to monitor for zoonotic diseases.